Lesson 2

Preparing the culture medium

Culture medium is repaired in a bottle according to the following recipe:

    For 1 liter water add:

16g Sodium Bicarbonate (NaHCO3)

2g Potassium Nitrate (KNO3)

1g Sodium Chloride (NaCl)

0.1g Potassium Dihydrogenate Phosphate (KH2PO4)

0.1g Iron Sulfate (FeSO4)

0.1g Magnesium Sulfate (MgSO4)

2. Mix until all the ingredients are dissolved.

3. Examine ph level. When ph level reaches a level of 8-8.5 – Spirulina is added.

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